Population study of horizontal cells in live carp retinas using microinjections of horseradish peroxidase

In order to randomly sample horizontal cells (HCs) of carp retina, horseradish peroxidase (HRP) was delivered into axon terminals by means of vitreally inserted micropipettes in live fish. Survival time was extended to several hours to permit label diffusion into HC somata. A function measuring the relative complexity of cell contours was defined in order to help classify HCs according to their morphological types. The histogram of measurement from 313 HCs showed three modes fitting normal distributions. The numbers of cells in each group were 267, 36 and 10, respectively, representing a relative population of 85.3% H1s, 11.5% H2s and 3.2% H3s. It is suggested that cone HC somata are extended in a monolayer constituted by predominant type-H1 with interspersed groups of both type-H2 and type-H3 cells.