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Microbial contamination associated with mascara use.

Latricia D Pack¹, M Gary Wickham, Rebecca A Enloe

¹Northeastern State University Oklahoma College of Optometry, Tahlequah, Oklahoma 74464, USA. packl@nsuok.edu

Optometry (St. Louis, Mo.)

|October 17, 2008

Summary

Daily mascara use can lead to microbial contamination. This study found significant bacterial and fungal growth in 36.4% of mascara tubes after three months, suggesting a need for regular replacement.

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Area of Science:

Ophthalmology
Microbiology
Cosmetic Science

Background:

Human eyelashes harbor microbial organisms.
Mascara application can introduce microbes into mascara tubes.
This study examined mascara contamination in a real-world setting.

Purpose of the Study:

To investigate microbial contamination in mascara.
To assess contamination after daily use for three months.

Main Methods:

Forty women used one of two non-waterproof mascara brands daily for three months.
Mascara tubes were collected and cultured for microbial growth.
Participants were aged 18-39, and tubes were unexpired.

Main Results:

Microbial growth was detected in 36.4% of mascara tubes after three months.
Common contaminants included Staphylococcus epidermidis, Streptococcus species, and fungi.
33 out of 40 participants completed the study.

Conclusions:

Daily mascara use for over three months is associated with microbial contamination.
A recommendation of a maximum three-month usage for mascara is advised.
Further research may indicate more frequent replacement intervals.