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Related Experiment Video

Updated: Jun 28, 2026

Purification of Progenitors from Skeletal Muscle

Purification of Progenitors from Skeletal Muscle

Published on: March 16, 2011

Sirt1 increases skeletal muscle precursor cell proliferation.

Christopher R Rathbone¹, Frank W Booth, Simon J Lees

¹Department of Biomedical Sciences, University of Missouri-Columbia, Columbia, MO 65211, USA. rathbone@email.arizona.edu

European Journal of Cell Biology

|October 17, 2008

Summary

Sirtuin 1 (Sirt1) promotes muscle precursor cell (MPC) proliferation, crucial for muscle growth and repair. Understanding Sirt1

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Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Published on: February 16, 2017

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Last Updated: Jun 28, 2026

Purification of Progenitors from Skeletal Muscle

Purification of Progenitors from Skeletal Muscle

Published on: March 16, 2011

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Published on: February 16, 2017

Area of Science:

Muscle biology
Cellular and molecular biology
Aging research

Background:

Aging leads to skeletal muscle mass loss and impaired regeneration.
Muscle precursor cell (MPC) proliferation is key to muscle maintenance and repair.

Purpose of the Study:

To investigate the role of NAD+-dependent histone deacetylase Sirtuin 1 (Sirt1) in regulating MPC proliferation.
To explore the influence of oxygen levels on MPC proliferation and Sirt1 expression.

Main Methods:

Over-expressed Sirt1 in MPCs and assessed proliferation markers (BrdU, cell number, PCNA, pRb phosphorylation).
Measured changes in cyclin-dependent kinase inhibitors (p21, p27) expression.
Cultured MPCs at varying oxygen concentrations (5% vs. 20% O2) and modulated Sirt1 activity with resveratrol or nicotinamide.

Main Results:

Sirt1 over-expression increased MPC proliferation and cell cycle progression.
Lowering oxygen to 5% increased both MPC proliferation and Sirt1 expression.
Sirt1 activation (resveratrol) enhanced proliferation, while inhibition (nicotinamide) reduced it.

Conclusions:

Sirt1 directly promotes MPC proliferation, offering a potential therapeutic target.
Oxygen levels influence Sirt1 expression and MPC proliferation.
Findings have implications for combating age-related muscle loss and enhancing muscle repair.