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Related Concept Videos

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Cancer Prevention

Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
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Radical Autoxidation01:20

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Cooperative Binding of Transcription Regulators

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Related Experiment Video

Updated: Jun 28, 2026

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
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Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro

Published on: July 5, 2017

RNA binding to antioxidant flavonoids.

Sh Nafisi1, A Shadaloi, A Feizbakhsh

  • 1Department of Chemistry, Azad University, Central Tehran Branch (IAUCTB), Tehran 1467686831, Iran. drsnafisi@yahoo.com

Journal of Photochemistry and Photobiology. B, Biology
|October 17, 2008
PubMed
Summary

This study investigated flavonoid interactions with RNA, finding that apigenin, naringin, and morin bind to RNA. Apigenin showed the highest binding affinity, with RNA structure remaining stable.

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Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
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Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
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Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
09:50

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Published on: August 14, 2019

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Natural Products Chemistry

Background:

  • Flavonoids are natural polyphenols with known bioactivities, including anticancer and antiviral effects.
  • While flavonoid-DNA interactions are studied, their binding to RNA is less understood.
  • RNA's role in biological processes necessitates understanding its interactions with small molecules.

Purpose of the Study:

  • To investigate the binding interactions between three flavonoids (morin, apigenin, naringin) and yeast RNA.
  • To determine the binding modes, constants, and stability of RNA in flavonoid-RNA complexes.
  • To compare the binding affinities of different flavonoids to RNA.

Main Methods:

  • Utilized Fourier-transform infrared (FTIR) and UV-visible spectroscopy.
  • Examined interactions in aqueous solution at physiological conditions.
  • Employed constant RNA concentration with varying flavonoid/RNA ratios.

Main Results:

  • Spectroscopic data confirmed significant binding of all three flavonoids to RNA.
  • Binding constants were determined: K(morin) = 9.150 x 10^3 M⁻¹, K(apigenin) = 4.967 x 10^4 M⁻¹, K(naringin) = 1.144 x 10^4 M⁻¹.
  • The binding affinity order was established as apigenin > naringin > morin.

Conclusions:

  • Flavonoids exhibit notable binding to RNA, with apigenin demonstrating the strongest affinity.
  • Flavonoid complexation did not induce changes in RNA secondary structure, preserving its A-family conformation.
  • These findings contribute to understanding flavonoid-RNA interactions with potential biomedical implications.