Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

On the mechanism of the decrease in cerebellar cyclic GMP content elicited by opiate receptor agonists.

G Biggio, A Guidotti, E Costa

    Naunyn-Schmiedeberg'S Archives of Pharmacology
    |January 1, 1977
    PubMed
    Summary

    Morphine and related drugs reduce cyclic guanosine monophosphate (cGMP) in the rat cerebellum. This effect, mediated by striatal opiate receptors, suggests a link between opioid signaling and cerebellar function.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Behavioral and molecular neuroepigenetic alterations in prenatally stressed mice: relevance for the study of chromatin remodeling properties of antipsychotic drugs.

    Translational psychiatry·2016
    Same author

    DNA-methyltransferase1 (DNMT1) binding to CpG rich GABAergic and BDNF promoters is increased in the brain of schizophrenia and bipolar disorder patients.

    Schizophrenia research·2014
    Same author

    Toward the identification of peripheral epigenetic biomarkers of schizophrenia.

    Journal of neurogenetics·2014
    Same author

    Increased binding of MeCP2 to the GAD1 and RELN promoters may be mediated by an enrichment of 5-hmC in autism spectrum disorder (ASD) cerebellum.

    Translational psychiatry·2014
    Same author

    DNA-methylation gene network dysregulation in peripheral blood lymphocytes of schizophrenia patients.

    Schizophrenia research·2013
    Same author

    Surgical correction of a saccular descending aortic aneurysm.

    HSR proceedings in intensive care & cardiovascular anesthesia·2013

    Area of Science:

    • Neuroscience
    • Pharmacology
    • Biochemistry

    Background:

    • Cyclic guanosine monophosphate (cGMP) plays a role in neuronal signaling.
    • Opioid receptors are known to modulate various brain functions.

    Purpose of the Study:

    • To investigate the effect of morphine and related analgesics on cerebellar cGMP levels.
    • To determine the brain regions and receptor systems involved in morphine's action on cerebellar cGMP.

    Main Methods:

    • Administration of morphine, dextromoramide, and vimonol R2 to rats.
    • Measurement of cGMP content in rat cerebellar cortex and deep cerebellar nuclei.
    • Intrastriatal and intracerebellar injections of morphine.
    • Administration of naltrexone and apomorphine to assess receptor involvement.

    Related Experiment Videos

  • Pretreatment with 3-acetylpyridine to investigate the role of climbing fibers.

    • Main Results:

    • Analgesic doses of morphine, dextromoramide, and vimonol R2 decreased cerebellar cGMP.
    • Intrastriatal morphine, but not intracerebellar, reduced cerebellar cGMP.
    • The effect of morphine was blocked by apomorphine (dopaminergic agonist) but not naltrexone (opiate antagonist).
    • 3-acetylpyridine pretreatment did not prevent the reduction of cerebellar cGMP by morphine.

    Conclusions:

    • Activation of striatal opiate receptors by morphine leads to a decrease in cerebellar cGMP.
    • This reduction in cGMP is likely mediated by a decrease in excitatory mossy fiber input to the cerebellum.
    • The findings suggest a novel pathway linking striatal opioid activity to cerebellar function.