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Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
Published on: June 17, 2019
Combined multilocus short-sequence-repeat and mycobacterial interspersed repetitive unit-variable-number
Virginie C Thibault1, Maggy Grayon, Maria Laura Boschiroli
1INRA, UR1282, Infectiologie Animale, Santé Publique, Nouzilly, France.
Abstract:
Short-sequence-repeat (SSR) sequencing was applied to 127 Mycobacterium avium subsp. paratuberculosis isolates typed by mycobacterial interspersed repetitive unit-variable-number tandem repeats (MIRU-VNTR) and IS900 restriction fragment length polymorphism (RFLP). Combined MIRU-VNTR and SSR typing followed by secondary IS900 RFLP typing is an improved approach to high-resolution genotyping of this pathogen.
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