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Related Experiment Videos

Development and application of a multiple typing system for Clostridium difficile.

D E Mahony1, J Clow, L Atkinson

  • 1Department of Microbiology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada.

Applied and Environmental Microbiology
|July 1, 1991
PubMed
Summary
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Differentiating Clostridium difficile strains requires multiple methods. Combining bacteriocin, plasmid, and phage typing, along with cytotoxin analysis, significantly improves strain identification capabilities.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Clostridium difficile is a significant cause of healthcare-associated infections.
  • Accurate strain differentiation is crucial for epidemiological studies and infection control.
  • Existing typing methods for Clostridium difficile have limitations in typeability.

Purpose of the Study:

  • To evaluate the effectiveness of bacteriocin, bacteriophage, and plasmid typing for differentiating Clostridium difficile strains.
  • To determine the optimal combination of typing techniques for achieving high strain typeability.
  • To assess the added value of cytotoxin production analysis in strain differentiation.

Main Methods:

  • Screening of 400 Clostridium difficile isolates to establish a bacteriocin-producing strain typing set.

Related Experiment Videos

  • Typing of 114 Clostridium difficile isolates using bacteriocin, plasmid, and bacteriophage techniques.
  • Analysis of plasmid profiles and cytotoxin production in non-typeable isolates.

    • Main Results:

    • Bacteriocin typing identified 31 patterns among 40% of isolates; plasmid typing revealed 20 patterns in 59% of isolates.
    • Combined bacteriocin and plasmid typing achieved 77% typeability.
    • Phage typing increased typeability by 1.8%; cytotoxin analysis further improved differentiation to 84-86%.

    Conclusions:

    • No single typing method is sufficient for differentiating Clostridium difficile strains.
    • A combination of bacteriocin, plasmid, and potentially phage typing, supplemented by cytotoxin analysis, is necessary for acceptable strain differentiation.
    • Integrated molecular and phenotypic typing strategies enhance epidemiological surveillance of Clostridium difficile.