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Related Concept Videos

Neurulation01:30

Neurulation

Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the anterior...

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Increasing Durability of Dissociated Neural Cell Cultures Using Biologically Active Coralline Matrix
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Aragonite crystalline matrix as an instructive microenvironment for neural development.

H Peretz1, P Blinder, L Segal

  • 1Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva, 84105, Israel.

Journal of Tissue Engineering and Regenerative Medicine
|October 17, 2008
PubMed
Summary
This summary is machine-generated.

Marine coral skeletons promote neural cell growth by providing essential calcium ions and supportive architecture. This biomaterial shows promise for nervous tissue engineering applications.

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Area of Science:

  • Biomaterials Science
  • Neuroscience
  • Tissue Engineering

Background:

  • Mimicking natural cell-matrix interactions is crucial for neuroscience and nervous tissue engineering.
  • Marine aragonite matrices previously showed potential for promoting neural cell growth.
  • Understanding neural cell-material interactions requires examining scaffold architecture and activity.

Purpose of the Study:

  • To investigate the mechanism of neural cell-material interactions with marine aragonite scaffolds.
  • To explore the role of three-dimensional (3D) surface architecture and matrix activity.
  • To determine if neurons and astrocytes can utilize calcium ions from the biomatrix.

Main Methods:

  • Developed a cloning technique for hydrozoan Millepora dichotoma to label skeletons with calcein or (45)Ca(2+).
  • Cultured hippocampal neuronal cells on labelled aragonite matrices.
  • Compared cell survival and calcium uptake on labelled aragonite, gold-coated coral skeletons, and with extracellular calcium chelation (EGTA).

Main Results:

  • Neurons and astrocytes successfully took up calcium ions (Ca(2+)) from the aragonite biomatrix, enhancing uptake when extracellular calcium was limited.
  • Scaffold architecture played a role in neural cell survival, independent of calcium uptake.
  • The effects of scaffold architecture and chemistry were more significant for neurons than for astrocytes.

Conclusions:

  • Neural cells can actively translocate calcium from biomaterials, influencing cell behavior and survival.
  • The 3D architecture and calcium content of marine aragonite scaffolds are key factors in supporting neural cells.
  • These findings highlight the potential of aragonite-based biomaterials for advanced neural tissue engineering.