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Updated: Jun 28, 2026

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

Published on: March 31, 2013

Fluid dynamics in zebrafish Kupffer's vesicle.

Noriko Okabe¹, Bo Xu, Rebecca D Burdine

¹Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|October 17, 2008

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Zebrafish Kupffer's vesicle cilia generate a tilted circular flow, not a simple leftward current. This unique fluid dynamics may explain how left-right asymmetry is established in developing zebrafish.

Area of Science:

Developmental Biology
Biophysics

Background:

Cilia motility and leftward nodal flow are implicated in left-right symmetry breaking in mice.
Zebrafish Kupffer's vesicle is considered analogous to the mouse node, but its internal architecture and fluid dynamics remain unclear.

Purpose of the Study:

To investigate the fluid dynamics within the zebrafish Kupffer's vesicle.
To understand the role of cilia and cell orientation in establishing left-right asymmetry in zebrafish.

Main Methods:

Analysis of cilia orientation and movement within Kupffer's vesicle.
Tracking bead movements to determine fluid flow patterns.
Histological analysis of cell orientation.

Main Results:

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Related Experiment Videos

Last Updated: Jun 28, 2026

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

Published on: March 31, 2013

Manual Drainage of the Zebrafish Embryonic Brain Ventricles

Manual Drainage of the Zebrafish Embryonic Brain Ventricles

Published on: December 16, 2012

AFM and Microrheology in the Zebrafish Embryo Yolk Cell

AFM and Microrheology in the Zebrafish Embryo Yolk Cell

Published on: November 29, 2017

Cells in Kupffer's vesicle possess posteriorly pointed cilia that rotate clockwise.

A net circular flow, tilted with respect to the dorsal-ventral axis, was observed.

Local flow direction varies within the vesicle, potentially influenced by anterior-dorsal cell orientation.

Conclusions:

Zebrafish Kupffer's vesicle exhibits complex, tilted circular fluid flow, distinct from the mouse node.
The observed flow dynamics suggest a novel mechanism for initiating left-right asymmetry in zebrafish development.