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Updated: Jun 28, 2026
06:24
Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
Optimizing production of serially diluted compounds and distribution to multiple targets.
1Research and Development, GlaxoWellcome, USA.
Journal of Automated Methods & Management in Chemistry
|October 18, 2008
Summary
A new robotic system automates compound serial dilution and assay plate preparation for drug discovery. This high-throughput system efficiently produces over 200 serial dilution assay plates daily.
Area of Science:
- Biotechnology
- Drug Discovery
- Laboratory Automation
Background:
- Drug discovery requires assessing compound selectivity across multiple targets.
- Manual serial dilution and plate preparation are time-consuming and labor-intensive.
- There is a need for automated solutions to increase throughput and accuracy.
Purpose of the Study:
- To establish a robotic system for automated serial dilution and assay plate production.
- To improve the efficiency and accuracy of compound screening processes.
- To support multiple-target compound selectivity programs.
Main Methods:
- Integration of a Genesis RSP 150 with a Zymate Laboratory Automation System XP.
- Development of a robotic workflow for serial compound dilution.
- Automated distribution of diluted compounds to replicate assay plates using carousels and rapid plate handlers.
Main Results:
- The robotic system successfully automates serial dilution and assay plate preparation.
- High-throughput production of over 200 serial dilution assay plates per workday.
- Demonstrated speed and accuracy in plate preparation.
Conclusions:
- The established robotic system effectively addresses the need for high-throughput compound selectivity screening.
- Automation significantly enhances the efficiency of assay plate production.
- This system enables faster and more accurate compound evaluation in drug discovery programs.
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