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Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.

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Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye
07:44

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Automation photometer of Hitachi U-2000 spectrophotometer with RS-232C-based computer.

K S Kumar1, B S Lakshmi, G Pennathur

  • 1Sub-DIC Biotechnology Information Services Centre for Biotechnology Anna University Madras 600 025 India.

The Journal of Automatic Chemistry
|October 18, 2008
PubMed
Summary
This summary is machine-generated.

This study details interfacing a Hitachi U2000 spectrophotometer to a computer for data acquisition. The developed system enables efficient spectral analysis and plotting, validated with a cyanine dye experiment.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Computer Interfacing

Background:

  • Spectrophotometers like the Hitachi U2000 are crucial for chemical analysis.
  • Direct computer interfacing of such instruments can enhance data handling and analysis.
  • Existing methods may lack cost-effectiveness or ease of implementation.

Purpose of the Study:

  • To describe the interfacing of a Hitachi U2000 spectrophotometer to an IBM compatible computer.
  • To present the design of custom hardware and C programming language software for data acquisition.
  • To demonstrate the system's efficacy through experimental spectral analysis and data transformation.

Main Methods:

  • Hardware modification of readily available materials for RS-232C port interfacing.
  • Software development using the C programming language for data acquisition and control.
  • Experimental validation using the visible spectrum of a cyanine dye.
  • Data plotting and transformation to a wavenumber scale using custom C modules.

Main Results:

  • Successful interfacing of the Hitachi U2000 spectrophotometer with a personal computer.
  • Generation of spectral data for a cyanine dye, plotted via a computer-connected printer.
  • Capability to transform spectral data to the wavenumber scale using developed software modules.
  • Calculation of the overall efficiency of the developed interfacing system.

Conclusions:

  • The described method provides an effective and potentially cost-efficient way to interface a Hitachi U2000 spectrophotometer with a computer.
  • The developed hardware and software facilitate automated data acquisition and spectral analysis.
  • This approach enhances the utility of standard spectrophotometers in research and analytical laboratories.