Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis of deuterium labeled cholesterol and steroids and their use for metabolic studies.

Lipids·2016
Same author

Evaluation of automatically generated substructure identification rules from tandem mass spectra.

Journal of the American Society for Mass Spectrometry·2013
Same author

Applications of Fourier transform infrared microspectroscopy in studies of benign prostate and prostate cancer. A pilot study.

The Journal of pathology·2003
Same author

Simplex optimization of conditions for the determination of arsenic in environmental samples by using electrothermal atomic absorption spectrometry.

Talanta·1994
Same author

Spectrophotometric method for determination of sulfide with iron(III) and nitrilotriacetic acid by flow injection.

Talanta·1992
Same author

Systematic delineation of scan modes in multidimensional mass spectrometry.

Analytical chemistry·1990

Related Experiment Video

Updated: Jun 28, 2026

Measurement and Analysis of Atomic Hydrogen and Diatomic Molecular AlO, C2, CN, and TiO Spectra Following Laser-induced Optical Breakdown
09:40

Measurement and Analysis of Atomic Hydrogen and Diatomic Molecular AlO, C2, CN, and TiO Spectra Following Laser-induced Optical Breakdown

Published on: February 14, 2014

Versatile IEEE-488 data acquisition and control routines for a diode array spectrophotometer.

P M Shiundu1, A P Wade

  • 1Laboratory for Automated Chemical Analysis Chemistry Department University of British Columbia 2036 Main Mall. Vancouver British Columbia V6T 1Y6 Canada.

The Journal of Automatic Chemistry
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

This study presents simple data acquisition and control routines for the HP-8452A UV-visible diode array spectrophotometer. These routines enable full spectrum capture and absorbance measurements over time using an IBM compatible microcomputer.

More Related Videos

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Related Experiment Videos

Last Updated: Jun 28, 2026

Measurement and Analysis of Atomic Hydrogen and Diatomic Molecular AlO, C2, CN, and TiO Spectra Following Laser-induced Optical Breakdown
09:40

Measurement and Analysis of Atomic Hydrogen and Diatomic Molecular AlO, C2, CN, and TiO Spectra Following Laser-induced Optical Breakdown

Published on: February 14, 2014

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Laboratory Automation

Background:

  • UV-visible diode array spectrophotometers are essential laboratory tools.
  • Efficient data acquisition and instrument control are crucial for experimental success.

Purpose of the Study:

  • To develop simple data acquisition and control routines for the HP-8452A spectrophotometer.
  • To enable microcomputer interfacing for enhanced experimental control and data capture.

Main Methods:

  • Utilized Microsoft QuickBasic for programming.
  • Developed routines for interfacing an HP-8452A spectrophotometer with IBM PC/XT/AT compatible microcomputers.
  • Implemented functions for full spectrum capture and time-interval absorbance measurements.

Main Results:

  • Successfully created routines for capturing full spectra.
  • Enabled measurement of absorbance at specific wavelengths over preset time intervals.
  • Provided an option to calculate absorbance variance at each wavelength.

Conclusions:

  • The developed routines offer a straightforward method for controlling the HP-8452A spectrophotometer.
  • This facilitates automated data collection and analysis in laboratory settings.
  • The software enhances the utility of the spectrophotometer for kinetic and spectral studies.