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Related Concept Videos

Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
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Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
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Transportation of samples from the collection point to the laboratory, as well as storage and preservation techniques, are crucial for maintaining sample integrity and ensuring accurate and reliable test results.
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An evaluation of the Kemtek 1000 sample processor.

M J Wheeler1, L Waiters

  • 1Department of Chemical Pathology St Thomas' Hospital London SE1 7EH UK.

The Journal of Automatic Chemistry
|January 1, 1988
PubMed
Summary

The Kemtek 1000 Sample Processor demonstrates excellent precision (<1.0%) and accuracy (+/-5%) for laboratory assays. This automated system efficiently processes samples, reducing carry-over contamination and proving reliable for routine use.

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Area of Science:

  • Clinical laboratory automation
  • Biochemical assay development

Background:

  • Automated sample processing is crucial for high-throughput clinical laboratories.
  • Ensuring precision, accuracy, and minimizing carry-over are key performance indicators for laboratory automation.

Purpose of the Study:

  • To evaluate the performance of the Kemtek 1000 Sample Processor.
  • To assess its precision, accuracy, speed, reliability, and potential for carry-over contamination.

Main Methods:

  • The Kemtek 1000 Sample Processor was tested for precision and accuracy across various volumes.
  • Assay setup time and carry-over contamination were quantified using a two-probe system.
  • Protein adsorption to probe tubing was investigated, with mitigation strategies explored.

Main Results:

  • Precision was consistently better than 1.0%, and accuracy was within +/-5%.
  • A 100-tube assay could be set up in 15 minutes.
  • Carry-over was reduced to <0.01% with appropriate wash steps; protein adsorption was manageable.

Conclusions:

  • The Kemtek 1000 Sample Processor is a precise, accurate, and reliable instrument for laboratory assay setup.
  • The system's efficiency and manageable carry-over make it suitable for routine clinical use.
  • Minor issues encountered were resolved promptly, fostering staff confidence in the automated processor.