Computer-based automation of concentration measurements with ion-selective electrodes
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Updated: Jun 28, 2026

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
Published on: May 3, 2015
J A Ibañez1, L Victoria, R M Barzanallana
1Dept. Física Aplicada Facultad de Ciencias Químicas y Matemáticas Universidad de Murcia Murcia 30071 Spain.
Abstract:
An integrated computer system consisting of an expandable ionanalyzer and a PC has been used to automate concentration measurements with ion-selective electrodes (ISEs). The ionanalyzer determines ionic concentrations using a reference electrode coupled with an ISE. The measurements and calibrations are carried out in a thermostated sample changer equipped with a flow cell. The data obtained by the ionanalyzer are sent via a standard RS 232-C interface to a microcomputer. In this paper, we describe the automatic data acquisition system and the subsequent processing of the measurements. One program (Transorion) automatically collects the measurements carried out by the ionanalyzer, giving a real-time graphic representation of the measurement on the computer screen. A second program (Graforion) facilitates the management of the data stored by the first program, and listing and graphics of these can be obtained on the printer/plotter. The method has been used to study continuous concentration changes in an aqueous solution of potassium iodide.
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