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Published on: October 30, 2018
[Cortical neuron apoptosis induced by beta-amyloid peptide and protective effect of panoxadiol in mice]
Jing Zhou1, Bai-qiang Sun, Rui-lan Gao
1Department of Anatomy and Cell Biology, College of Medicine, Zhejiang University, Hangzhou 310058, China.
Objective:
To investigate the apoptosis of cortical neurons induced by beta-amyloid peptide (Abeta(1-40)) and the protective effect of panoxadiol.
Methods:
The Abeta(1-40) induced damage of primarily cultured mouse cortical neurons was examined with morphological observation, MTT assay, DNA agarose gel electrophoresis and Western-blot.
Result:
After 48 h treated with 12 mumol/L Abeta(1-40), the cortical neurons showed apoptotic characteristics: including decreased OD570 value in MTT assay, DNA cleavage fragment in electrophoresis and increased apoptotic cells. Western-blot showed that the expression of bcl-2 reduced significantly (P<0.05). Cell apoptosis was significantly attenuated in 40 mg/L panoxadiol treated group.
Conclusion:
Panoxadiol can protect cultured cortical neurons from apoptosis induced by Abeta(1-40) in mice.
