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Allergic Reactions

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Related Experiment Video

Updated: Jun 28, 2026

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

Published on: June 4, 2017

Array-based profiling of ragweed and mugwort pollen allergens.

G Gadermaier¹, N Wopfner, M Wallner

¹Christian Doppler laboratory for allergy diagnosis and therapy, Department of Molecular Biology, University of Salzburg, Salzburg, Austria.

|October 18, 2008

Summary

Diagnosing ragweed and mugwort pollen allergies is challenging due to cross-reactivity. Molecule-based diagnosis using microarray and ELISA helps differentiate these allergies and guides immunotherapy selection.

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Last Updated: Jun 28, 2026

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

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Published on: June 4, 2017

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Published on: November 19, 2016

Area of Science:

Allergy and Immunology
Molecular Diagnostics

Background:

Ragweed and mugwort pollen cause significant allergic reactions in late summer and autumn.
Distinguishing between these pollen allergies is difficult for allergists due to shared allergens and similar flowering times.

Purpose of the Study:

To assess sensitization patterns in weed-allergic patients using a comprehensive panel of purified allergens.
To compare results from allergen microarray and enzyme-linked immunosorbent assay (ELISA) for molecule-based allergy diagnosis.

Main Methods:

Purification and recombinant expression of eight ragweed and six mugwort pollen allergens.
Utilizing protein microarray slides and ELISA plates for allergen testing.
Analyzing serum IgE reactivity from 19 allergic individuals against single molecules.

Main Results:

All ragweed-allergic patients showed sensitization to Amb a 1, with 30% being monosensitized.
Art v 1 and Art v 3 were recognized by 89% of mugwort-allergic patients.
Significant cross-reactivity was noted, particularly with profilin and non-specific lipid transfer proteins, with comparable results between microarray and ELISA.

Conclusions:

Molecule-based diagnosis is crucial for differentiating ragweed and mugwort pollen allergies.
This approach aids in selecting the correct allergen source for effective specific immunotherapy.