Related Experiment Videos
Liquid chromatographic determination of acyclovir.
D Marini1, G Pollino, F Balestrieri
1Laboratorio Chimico Centrale delle Dogane, Roma.
Bollettino Chimico Farmaceutico
|March 1, 1991
Summary
A new, accurate High-Performance Liquid Chromatography (HPLC) method precisely quantifies acyclovir in various pharmaceutical forms. This method also detects guanine, a potential contaminant or degradation product, ensuring drug quality and safety.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- Acyclovir is a widely used antiviral medication.
- Accurate quantification of acyclovir and identification of impurities are crucial for pharmaceutical quality control.
Purpose of the Study:
- To develop and validate an easy and precise High-Performance Liquid Chromatography (HPLC) method for acyclovir determination.
- To incorporate the detection of guanine as a synthetic contaminant or degradation product.
Main Methods:
- Development of a High-Performance Liquid Chromatography (HPLC) method.
- Application of the method to lyophilized vials and ointments.
- Detection of guanine impurity.
Main Results:
- An easy and precise HPLC method for acyclovir quantification was successfully developed.
- The method demonstrated accuracy in analyzing acyclovir in lyophilized vials and ointments.
- The procedure effectively detects guanine, a potential impurity.
Conclusions:
- The developed HPLC method is simple, accurate, and suitable for routine pharmaceutical analysis of acyclovir.
- The method ensures the quality of acyclovir formulations by identifying critical impurities like guanine.