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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

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Updated: Jun 28, 2026

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

Published on: March 26, 2011

An efficient protocol for genomic DNA extraction from formalin-fixed paraffin-embedded tissues.

Sara Santos¹, Daniela Sá, Estela Bastos

¹Institute for Biotechnology and Bioengineering, Centre of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (CGB-UTAD/IBB), Quinta de Prados, Apdo 1013, 5001-801 Vila Real, Portugal.

Research in Veterinary Science

|October 18, 2008

Summary

This study introduces an advanced protocol for extracting high-quality DNA from formalin-fixed paraffin-embedded tissues (FFPET). This method enables successful genomic analysis of archival cat mammary tumor (CMT) samples, yielding amplifiable DNA up to 1182bp.

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DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

Published on: March 26, 2011

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Published on: August 21, 2016

Area of Science:

Genomics
Molecular Biology
Veterinary Pathology

Background:

Formalin-fixed paraffin-embedded tissues (FFPET) are a vast resource for genomic studies.
Archival FFPET are crucial for studying rare or historical diseases, like cat mammary tumors (CMT).
Existing DNA extraction methods from FFPET can be challenging for molecular analyses.

Purpose of the Study:

To develop and present an advanced protocol for high-quality DNA extraction from FFPET.
To validate the protocol using archival cat mammary tumor (CMT) samples.
To demonstrate the utility of the extracted DNA for downstream molecular studies.

Main Methods:

An advanced DNA extraction protocol was applied to formalin-fixed paraffin-embedded tissues (FFPET).
Thirty-six archival cat mammary tumor (CMT) samples were processed.
DNA quality was assessed via PCR amplification of single-copy fragments and subsequent sequencing.

Main Results:

High-quality genomic DNA (gDNA) was successfully extracted from FFPET of CMT samples.
The extracted DNA was suitable for PCR amplification.
Sequencing confirmed the ability to amplify DNA fragments up to 1182 base pairs.

Conclusions:

The developed protocol provides a reliable method for obtaining high-quality DNA from FFPET.
This technique enhances the potential for genomic research using archival veterinary samples, specifically CMT.
The protocol supports molecular studies requiring amplifiable and sequenceable DNA from challenging FFPET specimens.