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Updated: Jun 28, 2026
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
Published on: May 9, 2020
RNA bandages for photoregulating in vitro protein synthesis
Julia L Richards1, XinJing Tang, Anna Turetsky
1Department of Chemistry, University of Pennsylvania, 231 South 34th St., Philadelphia, PA 19104-6323, USA.
Abstract:
'RNA bandages' are composed of two 6-12-mer 2'-OMe RNA strands complementary to a mRNA target that are joined by a photocleavable linker. These tandem oligonucleotides typically exhibit much higher affinity for the mRNA than the individual strands. An RNA bandage with binding arms of different lengths and a 4-base gap blocked translation in vitro of GFP mRNA; subsequent near-UV irradiation restored translation. This provides a general method of photomodulating hybridization for a variety of oligonucleotide-based technologies.