Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Overview of the Vascular System01:20

Overview of the Vascular System

The vascular system comprises an extensive network of arteries, capillaries, and veins. The vascular system can be broadly divided into the blood and lymphatic systems. Typically, blood vessels can be categorized into three histological regions: tunica intima, tunica media, and tunica adventitia. The tunica intima consists of a single layer of endothelial cells attached to the basal lamina. Underlying the basal lamina is a connective tissue layer and an elastic lamina that gives stability and...
Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PDGF-BB as a potential biomarker for early diagnosis of postpartum hemorrhage.

Frontiers in global women's health·2026
Same author

Ciliary protein ARL13b detected on RBCs as a potential indicative biomarker of vaso-occlusive crisis and disease severity in SCD: a retrospective pilot study.

Journal of sickle cell disease·2026
Same author

Role of SCF/c-KIT axis in pericyte TNT-guided vessel branching.

Fluids and barriers of the CNS·2026
Same author

Retinopathy of Prematurity (ROP): Are We There Yet?

Medicina (Kaunas, Lithuania)·2026
Same author

Ciliary p75 neurotrophin receptor (p75NTR) facilitates the enrichment of exogenous amyloid beta (Aβ 1-42) peptide and promotes oxidative stress in human hippocampal astrocytes.

BMC molecular and cell biology·2026
Same author

DUSP5 Downregulation in Nucleus Accumbens Core Correlates with Cocaine-Induced Maladaptive Synaptic Plasticity.

Cells·2026

Related Experiment Video

Updated: Jun 28, 2026

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
09:53

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

Dusp-5 and Snrk-1 coordinately function during vascular development and disease.

Kallal Pramanik1, Chang Zoon Chun, Maija K Garnaas

  • 1Department of Pediatrics, Children's Research Institute Developmental Vascular Biology Program, Medical College of Wisconsin, Milwaukee, WI 53226, USA.

Blood
|October 18, 2008
PubMed
Summary

Dual-specific phosphatase-5 (Dusp-5) regulates vascular development by counteracting Snrk-1 kinase activity in angioblasts. This pathway is crucial for controlling cell populations and is implicated in human vascular anomalies.

More Related Videos

A Method for Labeling Vasculature in Embryonic Mice
09:58

A Method for Labeling Vasculature in Embryonic Mice

Published on: October 7, 2011

Related Experiment Videos

Last Updated: Jun 28, 2026

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
09:53

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

A Method for Labeling Vasculature in Embryonic Mice
09:58

A Method for Labeling Vasculature in Embryonic Mice

Published on: October 7, 2011

Area of Science:

  • Cellular Biology
  • Developmental Biology
  • Molecular Biology

Background:

  • Mitogen-activated protein kinases (MAPKs) are key regulators of cellular processes.
  • Phosphatases counteract MAPK activity to maintain cellular homeostasis.
  • Dual-specific phosphatase-5 (Dusp-5) is a member of the phosphatase family.

Purpose of the Study:

  • To investigate the role of Dusp-5 in vascular development in vivo.
  • To elucidate the interaction between Dusp-5 and Snrk-1 in angioblast development.

Main Methods:

  • Expression analysis of dusp-5 in angioblasts and vasculature.
  • Functional studies of Dusp-5 and Snrk-1 in angioblast populations.
  • Analysis of mutations in Dusp-5 and Snrk-1 in patients with vascular anomalies.

Main Results:

  • Dusp-5 is expressed in developing vasculature and angioblasts.
  • Dusp-5 counteracts the function of serine threonine kinase Snrk-1.
  • Dusp-5 and Snrk-1 collaboratively control angioblast populations in the lateral plate mesoderm, with Dusp-5 acting downstream of Snrk-1.

Conclusions:

  • The Snrk-1-Dusp-5 signaling pathway is essential for proper vascular development.
  • Dysregulation of this pathway, due to mutations in Dusp-5 or Snrk-1, is implicated in human vascular anomalies.