Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spermine uptake by rat intestinal brush-border membrane vesicles.

K Iseki1, M Kobayashi, K Miyazaki

  • 1Department of Pharmacy, Hokkaido University Hospital, School of Medicine, Sapporo, Japan.

Biochimica Et Biophysica Acta
|September 10, 1991
PubMed
Summary

Spermine uptake by rat intestinal vesicles is pH-dependent and involves both binding and saturable transport. This suggests a dual mechanism contributing to polyamine accumulation in the intestine.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of Perioperative Blood Transfusions in Renal Transplant Patients.

Transplantation proceedings·2018
Same author

Effect of Influenza Vaccine in Patients With Kidney Transplant.

Transplantation proceedings·2018
Same author

Time course of osteoporotic vertebral fractures by magnetic resonance imaging using a simple classification: a multicenter prospective cohort study.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2016
Same author

Predicting delayed union in osteoporotic vertebral fractures with consecutive magnetic resonance imaging in the acute phase: a multicenter cohort study.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2016
Same author

Plasma amino acid profiles are associated with insulin, C-peptide and adiponectin levels in type 2 diabetic patients.

Nutrition & diabetes·2014
Same author

Diffuse tract damage in the hemispheric deep white matter may correlate with global cognitive impairment and callosal atrophy in patients with extensive leukoaraiosis.

AJNR. American journal of neuroradiology·2012

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Biochemistry

Background:

  • Polyamines, such as spermine, play crucial roles in cellular processes.
  • Understanding the mechanisms of polyamine uptake in the intestine is essential for comprehending nutrient absorption and physiological regulation.

Purpose of the Study:

  • To investigate the mechanism of spermine uptake by isolated rat intestinal brush-border membrane vesicles.
  • To characterize the kinetic properties and influencing factors of spermine uptake.

Main Methods:

  • Studied spermine uptake using isolated rat intestinal brush-border membrane vesicles.
  • Analyzed uptake kinetics at different temperatures, pH, and osmolarities.
  • Investigated the influence of Na+ electrochemical gradient on spermine uptake.

Related Experiment Videos

Main Results:

  • Spermine uptake exhibited a biphasic pattern: rapid initial uptake followed by a slower phase.
  • Uptake was pH-dependent, with higher uptake at pH 7.5 compared to pH 6.5.
  • Spermine uptake was inversely proportional to extravesicular osmolarity at pH 7.5, indicating binding.
  • Kinetic analysis revealed a Km of 24.2 µM and Vmax of 206.1 pmol/mg protein/min at 37°C.
  • Uptake at 4°C was nonlinear, suggesting saturability and involvement of transport mechanisms.

Conclusions:

  • Spermine associates with intestinal brush-border membrane vesicles through both binding and a saturable transport process.
  • The interaction with the apical membrane contributes to spermine accumulation within the intestine.