[Cryopreservation of platelets after storage at normal temperature for 3 days and its clinical practice]

This study was purposed to investigate the feasibility of cryopreservation platelets under -80 degrees C after stored for 3 days at normal temperature and its clinical use. The platelet count, aggregation functions, adhesive ability, hypotonic shock reaction and CD62p expression of platelets preserved at normal temperature, cryopreserved on same day of collection and after storage for 3 days were detected, and possibility of using platelets cryopreserved after storage for 3 days in clinic was evaluated by comparison of contactable clinical cases. The results indicated that no significant differences in platelet count, hypotonic shock reaction and adhesive ability were found within 3 days of storage (p > 0.05); but differences in aggregation function and CD62p expression were significant (p < 0.05). As compared with platelets cryopreserved on same day of collection, the detected parameters such as platelet count, aggregation function, adhesive ability, hypotonic shock reaction, expression at storage for 3 days did not show significant differences (p > 0.015; CCI value in clinically use of them also did not show significant difference too (p > 0.05). It is concluded that the platelets after storage for 3 days can be cryopreserved, the efficiency of them in clinical use is not significant difference from platelets cryopreserved on same day of collection.