Related Experiment Video
Updated: Jun 28, 2026
Morphological and Functional Evaluation of Ribbon Synapses at Specific Frequency Regions of the Mouse Cochlea
Published on: May 10, 2019
[Ultra-structural study of the lateral portion of the auditory sensorial organ using a decalcification-free method]
Iván Méndez-Benegassi1, Almudena Trinidad, José Ramón García-Berrocal
1Grupo de Investigación Otológica, Servicio de Otorrinolaringología, Hospital Universitario Puerta de Hierro, Universidad Autónoma de Madrid, Madrid, España. ibenegassi@hotmail.com
Objective:
To explain the development of a new personal technique to study the spiral ligament and stria vascularis in Guinea pig cochleae by obtaining sample tissue without decalcification and to assess its validity for electron microscopy analysis.
Material And Method:
Samples were taken from five female Guinea pigs weighing 200-250 g and were fixed in glutaraldehyde and paraformaldehyde for analysis of the spiral ligament and stria vascularis ultrastructure by transmission electron microscopy.
Results:
All of the ultrastructure components in the spiral ligament and stria vascularis could be examined without the need for decalcification.
Conclusions:
Our method to obtain and analyze samples of cochlea side wall is valid, easy and faster.
