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Related Experiment Video

Updated: Jun 28, 2026

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells
14:04

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells

Published on: August 1, 2020

Optimization of flexor tendon tissue engineering with a cyclic strain bioreactor.

Jonathan Riboh1, Alphonsus K S Chong, Hung Pham

  • 1Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, CA 94304, USA.

The Journal of Hand Surgery
|October 22, 2008
PubMed
Summary

Intermittent cyclic strain in bioreactors promotes cell proliferation and collagen production in tendon tissue engineering. This method enhances cell growth and matrix synthesis, potentially accelerating in vitro development.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Mechanical stimulation is crucial for tendon healing and regeneration.
  • Optimizing bioreactor parameters is key for effective tendon tissue engineering.

Purpose of the Study:

  • To identify optimal bioreactor strain patterns for enhancing cell proliferation and collagen production.
  • To evaluate strain effects on cell morphology for flexor tendon tissue engineering.

Main Methods:

  • Cultured epitenon tenocytes (Es), sheath fibroblasts (Ss), bone marrow-derived mesenchymal stem cells (BMSCs), and adipoderived stem cells (ASCs).
  • Applied continuous and intermittent cyclic uniaxial strain patterns to assess effects on cell proliferation, collagen I production, and morphology.

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Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization
12:13

Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization

Published on: October 28, 2013

Related Experiment Videos

Last Updated: Jun 28, 2026

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells
14:04

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells

Published on: August 1, 2020

Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization
12:13

Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization

Published on: October 28, 2013

Main Results:

  • Adipoderived stem cells and sheath fibroblasts showed higher adhesion to bioreactor membranes.
  • Continuous cyclic strain inhibited proliferation but increased per-cell collagen production.
  • Intermittent cyclic strain (1 hour on/2 hours off) significantly increased proliferation and total collagen production in adipoderived stem cells and sheath fibroblasts.
  • Cyclic strain induced tenocyte-like cell alignment and nuclear elongation.

Conclusions:

  • Intermittent cyclic strain is effective in promoting cell proliferation and collagen I production.
  • Bioreactor-mediated mechanical stimulation can maintain tenocyte-like morphology in vitro.
  • Bioreactor use may expedite the in vitro phase of tendon tissue engineering.