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Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue
Published on: January 12, 2024
Isolation and characterization of mouse mesenchymal stem cells
1Transplantation Research Center, Samsung Biomedical Research Institute, Seoul, Republic of Korea.
Transplantation Proceedings
|October 22, 2008
Summary
Adipose tissue mesenchymal stem cells (MSCs) from C57BL/6 mice offer superior growth potential and easier culture for developing reliable mouse models in regenerative medicine research.
Area of Science:
- Stem cell biology
- Regenerative medicine
- Immunology
Background:
- Mesenchymal stem cells (MSCs) possess unique immunologic properties valuable for regenerative medicine.
- Establishing safe and effective human clinical applications requires robust animal models.
- Optimal methods and sources for obtaining mouse MSCs are not well-defined.
Purpose of the Study:
- To investigate mesenchymal stem cells (MSCs) from three different mouse strains and three distinct tissue sources.
- To identify the most suitable source and method for developing MSCs in a mouse model for future research.
Main Methods:
- MSCs were isolated from bone marrow, compact bone, and adipose tissue of BALB/c, C3H, and C57BL/6 mice.
- Cell morphology, immunophenotype (FACS), and growth potential (colony-forming unit fibroblasts) were analyzed.
- StemXVivo medium was used for cell culture.
Main Results:
- All isolated MSCs exhibited plastic-adherent, fibroblastic characteristics.
- Bone marrow MSC culture was less successful compared to compact bone and adipose tissue.
- MSCs were positive for CD29, CD44, CD105, Sca-1 and negative for CD34, TER-119, CD45, CD11b.
- Adipose tissue-derived MSCs demonstrated higher growth potential.
Conclusions:
- Culture of adipose tissue and compact bone-derived MSCs is more feasible than bone marrow-derived MSCs.
- C57BL/6 adipose tissue-derived MSCs (AT-MSCs) are a promising source for establishing MSC mouse models.
- These findings support the development of standardized mouse models for MSC research.
