Microcapsules through polymer complexation. II. By complex coacervation of polymers containing a low charge density

Area of Science:

• Polymer Chemistry
• Biomaterials Science
• Cell Encapsulation Technology

Background:

• Weak polyelectrolyte polymers based on hydroxyalkyl methacrylates were investigated.
• Relationships between polymer properties (ionizable group content, structure, molecular weight) and complex coacervation were established.
• Polymers with methacrylic acid and dimethylaminoethyl methacrylate functionalities were identified as promising for cell entrapment.

Purpose of the Study:

• To evaluate synthetic weak polyelectrolytes for mammalian cell encapsulation.
• To enhance the solubility and microcapsule-forming ability of basic polymers through quaternization.
• To assess the viability of encapsulated mammalian cells.

Main Methods:

• Synthesis of sparingly soluble synthetic weak polyelectrolytes.
• Investigation of complex coacervation for ionic complex formation.
• Quaternization of basic polymers to form quaternary ammonium groups.
• Encapsulation of guinea-pig erythrocytes and assessment of survival using the Drabkin's test.

Main Results:

• Established relationships between polymer characteristics and complex coacervation efficacy.
• Demonstrated improved solubility and microcapsule structural integrity of basic polymers post-quaternization.
• Reported approximately 41% survival of guinea-pig erythrocytes encapsulated for 4 days compared to control.

Conclusions:

• Selected synthetic weak polyelectrolytes show promise for mammalian cell encapsulation.
• Quaternization significantly enhances the performance of basic polymers for microcapsule formation.
• The developed system demonstrates moderate cell survival, warranting further investigation for biomaterial applications.