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Related Experiment Video

Updated: Jun 28, 2026

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
05:59

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Published on: May 19, 2023

Mitochondrial function in vivo: spectroscopy provides window on cellular energetics.

Catherine E Amara1, David J Marcinek, Eric G Shankland

  • 1Department of Radiology, University of Washington Medical Center, Seattle, WA 98195, USA.

Methods (San Diego, Calif.)
|October 22, 2008
PubMed
Summary

We developed new noninvasive methods using optical and magnetic resonance spectroscopy to assess mitochondrial function in human muscle. This allows for the diagnosis of metabolic and degenerative diseases and monitoring of treatment effectiveness.

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Area of Science:

  • Cellular Biology
  • Biophysics
  • Medical Diagnostics

Background:

  • Mitochondria are central to cellular energy production.
  • Mitochondrial dysfunction is implicated in numerous human diseases and aging.
  • Current methods for assessing mitochondrial function in vivo are limited.

Purpose of the Study:

  • To present novel analytical and technical methods for evaluating mitochondrial metabolism and function in human muscle.
  • To enable noninvasive quantification of mitochondrial oxidation and phosphorylation fluxes.
  • To identify specific in vivo defects associated with mitochondrial diseases.

Main Methods:

  • Utilized optical spectroscopy (OS) to measure hemoglobin and myoglobin oxygenation for mitochondrial respiration (O2 uptake).

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Measurement of Mitochondrial Respiration in Human and Mouse Skeletal Muscle Fibers by High-Resolution Respirometry
08:12

Measurement of Mitochondrial Respiration in Human and Mouse Skeletal Muscle Fibers by High-Resolution Respirometry

Published on: October 4, 2024

Related Experiment Videos

Last Updated: Jun 28, 2026

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
05:59

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Published on: May 19, 2023

Measurement of Mitochondrial Respiration in Human and Mouse Skeletal Muscle Fibers by High-Resolution Respirometry
08:12

Measurement of Mitochondrial Respiration in Human and Mouse Skeletal Muscle Fibers by High-Resolution Respirometry

Published on: October 4, 2024

  • Employed magnetic resonance spectroscopy (MRS) to quantify high-energy compounds (creatine phosphate, adenosine triphosphate) for mitochondrial phosphorylation (ATP flux).
  • Developed a combined functional test to assess mitochondrial energy coupling (ATP/O2), phosphorylation capacity (ATPmax), and oxidative capacity (O2max).

    • Main Results:

    • Successfully quantified key compounds in energy metabolism noninvasively.
    • Enabled determination of mitochondrial oxidation and phosphorylation fluxes in vivo.
    • Established a method to assess mitochondrial energy coupling and maximal capacities.

    Conclusions:

    • The novel OS and MRS methods provide a unique diagnostic probe for mitochondrial function and capacity in human muscle.
    • These tools can identify specific defects in vivo associated with metabolic and degenerative disorders.
    • The approach allows for monitoring the impact of interventions aimed at reversing mitochondrial dysfunction.