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Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
Published on: March 31, 2017
Four powdery mildew species with catenate conidia infect Galium: molecular and morphological evidence
Susumu Takamatsu1, Vasyl Heluta, Maria Havrylenko
1Graduate School of Bioresources, Mie University, 1577 Kurima-Machiya, Tsu 514-8507, Japan. takamatu@bio.mie-u.ac.jp
Abstract:
The Erysiphaceae are a group of obligately biotrophic fungi that cause powdery mildew disease of angiosperms. Due to their inability to be cultured on artificial media, the taxonomy of the Erysiphaceae has generally been based on the morphological characteristics of fresh and herbarium specimens. Thus, several morphological species with wide host ranges have long been maintained in this family, even though they clearly consist of several biological species. Erysiphe galii has been known as a powdery mildew of Galium spp. Recently, the former E. galii var. galii has been reassessed as Neoerysiphe galii and E. galii var. riedliana as Golovinomyces riedlianus, along with a taxonomic revision of the generic concept of the Erysiphaceae. The present study was conducted to evaluate the validity of the taxonomic revision of the two varieties of E. galii. During the course of this study, we found that the Galium powdery mildews consist of at least four different species, viz. Neoerysiphe galii, Golovinomyces orontii, G. riedlianus, and an unknown species collected in Argentina. The latter species is described as a new species, Golovinomyces calceolariae. The three species belonging to Golovinomyces are morphologically very similar to each other, i.e. the discrimination between them is rather difficult. The morphological differences of the three Golovinomyces species of Galium are discussed.
Insights
Powdery mildew fungi (Erysiphaceae) taxonomy is complex. This study reveals at least four distinct species infecting Galium plants, including a newly identified species, Golovinomyces calceolariae.
Area of Science:
- Mycology
- Plant Pathology
- Fungal Taxonomy
Background:
- Erysiphaceae fungi cause powdery mildew on angiosperms.
- Traditional taxonomy relies on morphology due to culturing difficulties.
- Previous classifications of Erysiphe galii have been revised, leading to new species and genera.
Purpose of the Study:
- To evaluate the validity of the taxonomic revision of Erysiphe galii varieties.
- To clarify the species diversity of powdery mildews on Galium spp.
Main Methods:
- Morphological analysis of fungal specimens.
- Comparison of Erysiphaceae species found on Galium plants.
- Taxonomic description of a new fungal species.
Main Results:
- The Galium powdery mildews comprise at least four species: Neoerysiphe galii, Golovinomyces orontii, Golovinomyces riedlianus, and a new species.
- The new species, collected in Argentina, is described as Golovinomyces calceolariae.
- Morphological differentiation among the three Golovinomyces species on Galium is challenging.
Conclusions:
- The taxonomic revision of Erysiphe galii is supported by the findings.
- Golovinomyces calceolariae represents a new addition to fungal diversity.
- Further research is needed to refine morphological distinctions within the Golovinomyces genus affecting Galium.
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