Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chronic Obstructive Pulmonary Disease III: Chronic Bronchitis Features

Chronic Obstructive Pulmonary Disease III: Chronic Bronchitis Features

Chronic bronchitis is a key phenotype of chronic obstructive pulmonary disease (COPD), characterized by airway-centered inflammation and mucus overproduction. It develops from long-term exposure to harmful particles or gases, most commonly cigarette smoke, which triggers a persistent inflammatory response.Cellular and Structural ChangesInflammation initially affects the large bronchi and later the smaller airways, with infiltration by immune cells, including neutrophils, macrophages, and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effects of p53-knockout in vascular smooth muscle cells on atherosclerosis in mice.

PloS one·2017

Same author

The Deubiquitinase USP37 Regulates Chromosome Cohesion and Mitotic Progression.

Current biology : CB·2015

Same author

The roles of akt isoforms in the regulation of podosome formation in fibroblasts and extracellular matrix invasion.

Cancers·2015

Same author

p53 Down regulates PDGF-induced formation of circular dorsal ruffles in rat aortic smooth muscle cells.

PloS one·2014

Same author

p53 in cell invasion, podosomes, and invadopodia.

Cell adhesion & migration·2014

Same author

A strategy for modulation of enzymes in the ubiquitin system.

Science (New York, N.Y.)·2013

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment

Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment

Published on: May 6, 2020

Phorbol ester-induced podosomes in normal human bronchial epithelial cells.

Helan Xiao¹, Rob Eves, Christina Yeh

¹Division of Cell and Molecular Biology, University Health Network Toronto General Research Institute, Toronto, Ontario, Canada.

Journal of Cellular Physiology

|October 22, 2008

Summary

Normal human airway epithelial cells can form podosome-like structures, which aid in cell migration and invasion, crucial for respiratory tissue repair and regeneration.

More Related Videos

P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives

P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives

Published on: June 15, 2020

Related Experiment Videos

Last Updated: Jun 28, 2026

Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment

Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment

Published on: May 6, 2020

P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives

P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives

Published on: June 15, 2020

Area of Science:

Cell Biology
Biochemistry
Regenerative Medicine

Background:

Basal cell spreading and migration are vital for airway epithelial repair after injury.
Podosomes, structures controlling cell migration and invasion, were previously identified in cancer and mesenchymal cells.

Purpose of the Study:

To investigate if normal human airway epithelial cells can form podosomes.
To understand the molecular mechanisms underlying cell migration and invasion in airway repair.

Main Methods:

Primary normal human bronchial epithelial cells and BEAS2B cells were treated with phorbol-12, 13-dibutyrate (PDBu).
PDBu's effect on cytoskeletal reorganization was analyzed using Z-stack scanning confocal microscopy.
Immunofluorescent staining identified molecular components of induced structures.
In situ zymography assessed matrix degradation capabilities.
Transwell chambers evaluated epithelial cell invasion.

Main Results:

PDBu induced the formation of podosome-like structures in airway epithelial cells.
These structures featured actin-rich columns and dorsal ruffles.
Podosomes degraded fibronectin-gelatin-sucrose matrix.
Epithelial cell invasion across Transwell chambers was enhanced.

Conclusions:

Podosomes and dorsal ruffles are formed by normal human airway epithelial cells.
These structures contribute to epithelial cell migration and invasion.
The findings suggest a role for podosomes in respiratory epithelial repair and regeneration.