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Related Experiment Video

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Live and ultraviolet-inactivated Lactobacillus rhamnosus GG decrease flagellin-induced interleukin-8 production in

Mariela Lopez1, Nan Li, Jasmeet Kataria

  • 1Department of Pediatrics, University of Florida, Gainesville, FL 32610-0296, USA.

The Journal of Nutrition
|October 22, 2008
PubMed
Summary
This summary is machine-generated.

UV-inactivated Lactobacillus rhamnosus GG (LGG) effectively reduces interleukin-8 (IL-8) production in intestinal cells, similar to live LGG. This offers a safe alternative for immune-compromised individuals seeking probiotic benefits.

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Published on: September 18, 2016

Area of Science:

  • Microbiology
  • Immunology
  • Gastroenterology

Background:

  • Probiotics, like Lactobacillus rhamnosus GG (LGG), are beneficial for gut health but pose risks to immunocompromised individuals.
  • Inactivated probiotics present a safer alternative, provided they retain therapeutic functions.
  • Interleukin-8 (IL-8) is a key inflammatory mediator in the gut.

Purpose of the Study:

  • To investigate if UV-inactivated LGG can downregulate IL-8 production in intestinal epithelial cells stimulated by flagellin.
  • To compare the efficacy of UV-inactivated LGG with live LGG in modulating inflammatory responses.
  • To elucidate the underlying molecular mechanisms, including NF-kappaB pathway involvement.

Main Methods:

  • Caco-2 intestinal cells were pretreated with live or UV-inactivated LGG.
  • Cells were stimulated with flagellin, a bacterial ligand.
  • IL-8 production was measured using ELISA.
  • NF-kappaB pathway activation was assessed via IkappaB and Ub-IkappaB expression and NF-kappaB localization.

Main Results:

  • Flagellin significantly increased IL-8 production.
  • Both live and UV-inactivated LGG markedly reduced flagellin-induced IL-8 production.
  • Both forms of LGG inhibited flagellin-induced NF-kappaB nuclear translocation.
  • Both forms of LGG reversed flagellin-induced IkappaB degradation, while UV-inactivated LGG also reduced Ub-IkappaB.

Conclusions:

  • UV-inactivated LGG is as effective as live LGG in reducing IL-8 production in intestinal epithelial cells.
  • Both live and inactivated LGG modulate the NF-kappaB pathway, offering a potentially safer probiotic alternative.
  • This finding supports the use of inactivated probiotics for managing gastrointestinal inflammation, especially in vulnerable populations.