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Increased expression of pigment epithelium-derived factor in allergic rhinitis

Hee Joon Kang1, Hyo-Hyun Park, Sung Won Chae

  • 1Department of Otorhinolaryngology-Head and Neck Surgery, Guro Hospital, Korea University College of Medicine, 80 Guro-dong, Guro-gu, Seoul, Korea 152-703.

Archives of Otolaryngology--Head & Neck Surgery
|October 22, 2008
PubMed
Abstract

Insights

Pigment epithelium-derived factor (PEDF) mRNA and protein are elevated in the nasal mucosa of allergic rhinitis patients. This suggests PEDF may contribute to chronic nasal inflammation in this condition.

Area of Science:

  • Immunology
  • Molecular Biology
  • Otorhinolaryngology

Background:

  • Allergic rhinitis involves chronic nasal inflammation.
  • The role of specific proteins like pigment epithelium-derived factor (PEDF) in this inflammation is not fully understood.

Purpose of the Study:

  • To investigate the expression and localization of pigment epithelium-derived factor (PEDF) mRNA and protein in the nasal mucosa.
  • To compare PEDF levels in patients with allergic rhinitis versus healthy controls.

Main Methods:

  • Used inferior turbinate mucosal samples from 10 allergic rhinitis patients and 10 controls.
  • Analyzed PEDF mRNA and protein expression using reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemistry.

Main Results:

  • Significantly increased expression of PEDF mRNA and protein was observed in allergic rhinitis patients compared to controls.
  • PEDF protein was localized to the epithelium and submucosal glands of the nasal mucosa.

Conclusions:

  • PEDF protein is expressed in the human nasal mucosa.
  • Elevated PEDF expression in allergic rhinitis suggests a potential role in chronic nasal inflammation.

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