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Related Experiment Video

Updated: Jun 28, 2026

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection
09:54

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

Published on: February 16, 2020

Nucleic acid sensor for M. tuberculosis detection based on surface plasmon resonance.

Nirmal Prabhakar1, Kavita Arora, Sunil K Arya

  • 1Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory, New Delhi, India.

The Analyst
|October 22, 2008
PubMed
Summary

Peptide nucleic acid (PNA) and deoxyribonucleic acid (DNA) probes immobilized on gold surfaces offer sensitive detection of Mycobacterium tuberculosis. PNA probes demonstrate superior performance in identifying single-base mismatches and exhibit a lower detection limit.

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Area of Science:

  • Biosensors and Nanotechnology
  • Molecular Diagnostics
  • Microbiology

Background:

  • Accurate detection of Mycobacterium tuberculosis is crucial for effective tuberculosis treatment.
  • Developing highly specific and sensitive biosensors is essential for rapid pathogen identification.
  • Peptide nucleic acids (PNA) offer unique advantages over DNA for nucleic acid recognition due to their enhanced binding affinity and stability.

Purpose of the Study:

  • To develop and characterize novel biosensors for Mycobacterium tuberculosis detection using immobilized PNA and DNA probes.
  • To evaluate the performance of PNA-based and DNA-based bioelectrodes for detecting complementary and mismatched targets.
  • To compare the sensitivity and specificity of PNA/Au and DNA/Au bioelectrodes for genomic DNA analysis.

Main Methods:

Related Experiment Videos

Last Updated: Jun 28, 2026

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection
09:54

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

Published on: February 16, 2020

  • Immobilization of cysteine-modified PNA and thiol-labeled DNA probes onto BK-7 gold-coated glass plates.
  • Characterization of DNA/Au and PNA/Au bioelectrodes using surface plasmon resonance (SPR), contact angle, atomic force microscopy (AFM), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV).
  • Hybridization studies to assess the detection of complementary, one-base mismatch, and non-complementary targets in Mycobacterium tuberculosis genomic DNA.

Main Results:

  • SPR analysis indicated higher PNA immobilization (252 millidegrees) compared to DNA (205 millidegrees).
  • Both PNA/Au and DNA/Au electrodes showed no binding with non-complementary targets.
  • The PNA/Au electrode demonstrated superior efficiency in detecting one-base mismatch sequences and a lower detection limit (1.0 ng/mL) compared to the DNA/Au electrode (3.0 ng/mL).
  • Kinetic analysis revealed association (k(a) = 8.5 x 10(4) M(-1)s(-1)) and dissociation (k(d) = 3.6 x 10(-3) s(-1)) rate constants for complementary sequences on the PNA/Au bioelectrode.

Conclusions:

  • The PNA/Au bioelectrode is a highly efficient platform for the sensitive and specific detection of Mycobacterium tuberculosis.
  • PNA probes offer improved performance over DNA probes, particularly for identifying sequence variations like single-base mismatches.
  • The developed SPR-based biosensor holds significant potential for rapid and reliable diagnosis of tuberculosis.