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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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Related Experiment Video

Updated: Jun 28, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

Ikaros regulates Notch target gene expression in developing thymocytes.

Sheila Chari1, Susan Winandy

  • 1Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|October 23, 2008
PubMed
Summary
This summary is machine-generated.

Ikaros protein acts as a crucial regulator for Notch target genes during T cell development. Loss of Ikaros function leads to abnormal Notch signaling in developing thymocytes, impacting T cell leukemia progression.

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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

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Last Updated: Jun 28, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

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Published on: February 28, 2017

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
05:48

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Published on: January 2, 2018

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
10:25

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

Area of Science:

  • Immunology
  • Developmental Biology
  • Molecular Biology

Background:

  • Ikaros and Notch signaling pathways are critical for T cell development.
  • Collaborative mutations affecting Ikaros and Notch promote T cell leukemogenesis.
  • The impact of these mutations on thymocyte development is not fully understood.

Purpose of the Study:

  • To investigate the role of Ikaros in regulating Notch target genes during T cell development.
  • To explore the consequences of Ikaros loss on thymocyte development and Notch signaling.
  • To determine the specificity of Ikaros's regulatory role in different thymocyte subsets.

Main Methods:

  • Analysis of Ikaros null T cell leukemia lines and primary thymocytes.
  • Assessment of Notch receptor cleavage and Notch target gene expression.
  • Comparison of Ikaros's role in double-negative, double-positive, and single-positive thymocytes.

Main Results:

  • Ikaros regulates expression of key Notch target genes (Hes1, Deltex1, pTa, Gata3, Runx1).
  • Ikaros loss causes Notch deregulation in leukemia cells and double-positive thymocytes.
  • This regulatory role of Ikaros is specific to double-positive and single-positive thymocytes.

Conclusions:

  • Ikaros and Notch have opposing roles in regulating a subset of Notch target genes.
  • Ikaros is essential for proper Notch pathway regulation in developing thymocytes.
  • Ikaros's function is critical for normal T cell development and preventing leukemogenesis.