The complete genome sequences for three simian enteroviruses isolated from captive primates
M Steven Oberste1, Xi Jiang, Kaija Maher
1Polio and Picornavirus Laboratory Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop G-17, Atlanta, GA 30333, USA. soberste@cdc.gov
Archives of Virology
|October 23, 2008
Summary
Researchers identified known and novel simian enteroviruses (SEVs) in macaques and sooty mangabeys with diarrhea. New types EV92 and EV103 were discovered, with EV103 belonging to an unclassified SEV species.
Area of Science:
- Virology
- Primate Health
- Infectious Diseases
Background:
- Enteroviruses are a significant cause of disease in various hosts, including non-human primates.
- Understanding enterovirus diversity in primate populations is crucial for disease management and zoonotic risk assessment.
- Previous studies have identified several simian enteroviruses (SEVs) in different macaque and mangabey species.
Purpose of the Study:
- To detect and characterize enteroviruses in fecal samples from diarrheal non-human primates.
- To identify novel enterovirus types circulating in primate populations.
- To determine the genomic and phylogenetic relationships of newly identified SEVs.
Main Methods:
- Reverse transcription-polymerase chain reaction (RT-PCR) was employed for enterovirus detection.
- Partial genome sequencing was performed on detected enterovirus isolates.
- Complete genome sequencing was conducted for representative novel strains (SV46, EV92, EV103).
Main Results:
- Simian enteroviruses SV6, SV19, and SV46 were detected in fecal specimens.
- Two novel enterovirus types, EV92 and EV103, were identified in rhesus macaques, pigtail macaques, and sooty mangabeys.
- Genomic analysis revealed that SV46 and EV92 belong to the Human enterovirus A species, while EV103 represents a new unclassified species.
Conclusions:
- The study expands the known diversity of simian enteroviruses.
- EV92 and EV103 represent newly discovered enterovirus types with implications for primate health surveillance.
- EV103's classification highlights the existence of uncharacterized enterovirus species within primate reservoirs.
Related Concept Videos
02:51Multi-species Conserved Sequences
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
02:33Retroviruses
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...