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Determination

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...

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Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

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Differential requirements for Fgf3 and Fgf8 during mouse forebrain development.

Thomas Theil¹, Elena Dominguez-Frutos, Thomas Schimmang

¹Centres for Neuroscience Research and Integrative Physiology, University of Edinburgh, Edinburgh, Scotland.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|October 23, 2008

Summary

Fibroblast Growth Factor 3 (Fgf3) is not essential for mouse forebrain development, unlike in zebrafish. Fgf8 plays a critical role in telencephalon size and patterning in mice.

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Area of Science:

Developmental biology
Neuroscience
Genetics

Background:

Fibroblast Growth Factors (Fgfs) are crucial for vertebrate telencephalon formation and patterning.
Fgf8 regulates telencephalon size and signaling center development in zebrafish and mice.
Fgf3 and Fgf8 exhibit combinatorial functions in zebrafish forebrain development.

Purpose of the Study:

To investigate the role of Fgf3 in mouse telencephalon development.
To examine the combined functions of Fgf3 and Fgf8 in mouse forebrain development.

Main Methods:

Analysis of Fgf3 null mouse mutants.
Analysis of mouse embryos lacking both Fgf3 and Fgf8 in the forebrain.
Assessment of telencephalic marker gene expression.

Main Results:

Fgf3 null mutants display normal forebrain development and gene expression.
Double mutants (Fgf3 and Fgf8) show reduced forebrain size compared to Fgf8 mutants, but no additional gene expression changes.
Fgf3 is not required for mouse telencephalon development, contrasting with its role in zebrafish.

Conclusions:

Fgf3 does not play a significant role in mouse forebrain development.
Fgf8 is essential for mouse telencephalon development, controlling its size and patterning.
Species-specific differences exist in the roles of Fgf3 and Fgf8 during forebrain development.