Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 28, 2026

Microfabricated Platforms for Mechanically Dynamic Cell Culture
15:21

Microfabricated Platforms for Mechanically Dynamic Cell Culture

Published on: December 26, 2010

Microvalve-assisted patterning platform for measuring cellular dynamics based on 3D cell culture.

Minseok S Kim1, Wonhye Lee, Yu Chang Kim

  • 1Department of Bio and Brain Engineering, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Korea.

Biotechnology and Bioengineering
|October 24, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Detection and prognostic role of circulating cancer-associated fibroblasts in the blood of melanoma patients.

Frontiers in cell and developmental biology·2026
Same author

Deciphering the Heterogeneity of Cancer-Associated Fibroblasts in Prostate Cancer: From Stromal Biology to Clinical Translation.

Cancers·2026
Same author

Biologically inspired microlens array camera for high-resolution wide field-of-view imaging.

Nature communications·2026
Same author

Circulating Tumor Cell-based Molecular Responses Stratify EGFR-TKI Efficacy in Patients with EGFR-Mutant Lung Cancer.

Cancer research and treatment·2026
Same author

Ultrasensitive Detection of Multiple Foodborne Pathogens Using CRISPR-Cas12a on a Finger-Actuated Microfluidic Device Integrated with a Modular Pressurizing Pump.

Analytical chemistry·2025
Same author

CD45⁺ hybrid circulating cells may reflect tumor-immune interactions and serve as transcriptomic indicators of metastatic potential in prostate cancer.

Theranostics·2025

A novel microfluidic platform enables 3D cell culture and drug screening by creating precise drug gradients. This microvalve-assisted patterning (MAP) system reduces drug usage and assay time for more efficient drug discovery.

Area of Science:

  • Biomedical Engineering
  • Drug Discovery
  • Cell Biology

Background:

  • Developing reliable 3D cell culture and assay platforms is crucial for accurate drug discovery.
  • Existing methods often lack precise control over cellular microenvironments and drug delivery.
  • There is a need for integrated systems that support both 3D cell culture and dynamic cellular assays.

Purpose of the Study:

  • To introduce a microfluidic platform, termed microvalve-assisted patterning (MAP), for 3D cell culture and cell-based assays.
  • To demonstrate the platform's capability in generating linear drug concentration gradients for studying cellular dynamics.
  • To validate the platform's utility in drug discovery through hepatotoxicity testing.

Main Methods:

  • Fabrication of the MAP platform using multilayer soft lithography.

More Related Videos

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
05:57

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Related Experiment Videos

Last Updated: Jun 28, 2026

Microfabricated Platforms for Mechanically Dynamic Cell Culture
15:21

Microfabricated Platforms for Mechanically Dynamic Cell Culture

Published on: December 26, 2010

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
05:57

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

  • Integration of microvalves for precise control of fluid exchange and cell-matrix patterning.
  • Utilizing the platform for 3D cell culture and real-time monitoring of cellular responses to drug gradients.
  • Performing hepatotoxicity tests using human hepatocellular liver carcinoma cells (HepG2).

    • Main Results:

    • The MAP platform successfully enabled 3D cell culture and stable media exchange without disrupting the cell-matrix scaffold.
    • Linear drug concentration gradients were accurately generated within the microfluidic channels.
    • Real-time monitoring of HepG2 cell morphology under varying drug concentrations provided dynamic insights.
    • Compared to MTT assays, the MAP platform reduced drug amount by 10-fold and assay time by 3-fold.

    Conclusions:

    • The microvalve-assisted patterning (MAP) platform offers a robust solution for integrated 3D cell culture and cell-based assays.
    • This technology enhances the efficiency and reliability of drug discovery by enabling precise control over microenvironments and reducing resource consumption.
    • The MAP platform facilitates dynamic cellular studies and toxicity assessments, paving the way for improved drug development processes.