Related Experiment Video
Updated: Jun 28, 2026
09:05
Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
Published on: March 11, 2020
Systemic Modulation of Gene Expression in Tomato by Trichoderma hamatum 382
Phytopathology
|October 24, 2008
Summary
Trichoderma hamatum 382 protects tomato plants against bacterial spot disease. This beneficial fungus induces systemic changes in plant gene expression related to stress and metabolism, enhancing disease resistance.
Area of Science:
- Plant Pathology
- Microbiology
- Molecular Biology
Background:
- Bacterial spot, caused by Xanthomonas euvesicatoria, is a significant disease affecting tomato crops.
- Trichoderma hamatum 382 is a potential biocontrol agent for plant diseases.
Purpose of the Study:
- To investigate the mechanism by which Trichoderma hamatum 382 induces disease resistance in tomato plants.
- To analyze the effect of T. hamatum 382 on gene expression in tomato leaves.
Main Methods:
- Tomato plants were treated with a peat mix inoculated with Trichoderma hamatum 382.
- High-density oligonucleotide microarrays were used to analyze gene expression in leaves before pathogen inoculation.
- Differential gene expression analysis was performed to identify modulated genes.
Main Results:
- Trichoderma hamatum 382 provided significant protection against bacterial spot disease.
- 45 genes were differentially expressed in tomato leaves treated with T. hamatum 382.
- Induced genes were associated with stress responses, RNA, DNA, protein metabolism, and extensin proteins.
Conclusions:
- Trichoderma hamatum 382 induces systemic changes in tomato plant physiology and enhances disease resistance.
- The biocontrol mechanism involves the modulation of stress and metabolism-related genes, rather than traditional systemic acquired resistance markers.
Related Concept Videos
01:17Gene Regulation During Sporulation
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
02:04Regulation of Transpiration by Stomata
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...