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Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
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Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

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Pathological Variations Within Xanthomonas campestris pv. mangiferaeindicae Support Its Separation Into Three

N Ah-You, L Gagnevin, F Chiroleu

    Phytopathology
    |October 24, 2008
    PubMed

    Insights

    Bacterial black spot pathogen strains show distinct variations in pathogenicity and genetic makeup across different Anacardiaceae hosts. These findings support reclassifying Xanthomonas campestris pv. mangiferaeindicae into three new pathovars based on host range and symptoms.

    Area of Science:

    • Plant Pathology
    • Bacteriology
    • Genomics

    Background:

    • Bacterial black spot, caused by Xanthomonas campestris pv. mangiferaeindicae, significantly impacts mango production.
    • Xanthomonads infect various plant genera within the Anacardiaceae family, necessitating a deeper understanding of strain variations.

    Purpose of the Study:

    • To investigate pathological variations among Xanthomonas strains collected worldwide from Anacardiaceae hosts.
    • To determine the genetic divergence and host specificity of different pathogen groups.
    • To propose a revised taxonomic classification for Xanthomonas campestris pv. mangiferaeindicae.

    Main Methods:

    • Pathogenicity assays on mango (Mangifera indica) and cashew (Anacardium occidentale) leaves.
    • Morphological analysis of disease lesions.
    • Amplified Fragment Length Polymorphism (AFLP) for genome-wide genetic analysis.

    Main Results:

    • Three distinct pathogenicity groups were identified based on host multiplication and lesion morphology.
    • Group I strains (Old World) infected mango and cashew; Group II (Brazil) infected cashew but not mango.
    • AFLP data revealed genetic distinctness, correlating with existing X. axonopodis groups, supporting the proposed reclassification.

    Conclusions:

    • Pathological and genetic variations warrant the reclassification of X. campestris pv. mangiferaeindicae into three pathovars: X. axonopodis pv. mangiferaeindicae, X. axonopodis pv. anacardii, and X. axonopodis pv. spondiae.
    • Evidence suggests long-distance dissemination of the pathogen, likely via infected plant material.

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