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Related Experiment Video

Updated: Jun 28, 2026

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The phytopathogenic mollicute-insect vector interface: a closer look.

J Fletcher, A Wayadande, U Melcher

    Phytopathology
    |October 24, 2008
    PubMed
    Summary

    Spiroplasma citri transmission relies on its ability to cross insect barriers, linked to chromosomal rearrangements. A specific membrane protein appears crucial for adherence to leafhopper cells.

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    Area of Science:

    • Plant pathology
    • Insect microbiology
    • Molecular biology

    Background:

    • Spiroplasma citri is a phytopathogenic mollicute transmitted by leafhoppers.
    • Transmission involves the pathogen moving through insect tissues and barriers.

    Purpose of the Study:

    • To investigate the molecular basis of Spiroplasma citri transmission.
    • To identify factors enabling Spiroplasma citri to cross insect barriers.

    Main Methods:

    • Comparative molecular analysis of transmissible and nontransmissible Spiroplasma citri lines.
    • Identification and characterization of chromosomal rearrangements.
    • Cloning and analysis of a specific open reading frame encoding a membrane protein.
    • In vitro adhesion assays using cultured leafhopper cells and protease treatment.

    Main Results:

    • Transmissible Spiroplasma citri lines possess chromosomal rearrangements, including inversions and deletions, absent in nontransmissible lines.
    • A deleted region in transmissible strains contains an open reading frame encoding a 58 kDa integral membrane protein.
    • This protein shows similarity to adhesins of zoopathogenic mycoplasmas.
    • Spiroplasma adhesion to leafhopper cells is mediated by membrane proteins and inhibited by proteases.

    Conclusions:

    • Chromosomal rearrangements are associated with Spiroplasma citri transmissibility.
    • A specific membrane protein likely plays a key role in Spiroplasma adherence to and crossing of insect host barriers.
    • This research contributes to understanding phytopathogenic mollicute transmission mechanisms.

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