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Initial Cellular Interactions Between Thielaviopsis basicola and Tobacco Root Hairs.

M E Hood, H D Shew

|March 1, 1997

Summary

Thielaviopsis basicola establishes a hemibiotrophic parasitic relationship with tobacco root hairs, initially colonizing living cells before causing host cell death. The fungus penetrates dead root hairs but fails to establish infection.

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Area of Science:

Plant Pathology
Mycology
Plant-Microbe Interactions

Background:

Thielaviopsis basicola is a fungal pathogen affecting various plant species.
Understanding initial host-pathogen interactions is crucial for disease management.
Root hairs are primary infection sites for many soil-borne pathogens.

Purpose of the Study:

To microscopically examine the cellular events during Thielaviopsis basicola infection of tobacco root hairs.
To document the dynamic interaction and host cell responses.
To characterize the infection strategy of T. basicola.

Main Methods:

Time-course microscopic observation of Thielaviopsis basicola interacting with tobacco (Nicotiana tabacum) root hairs.
Documentation of fungal differentiation, host cell cytology, and penetration mechanisms.
Analysis of host responses including cytoplasmic streaming and callose deposition.

Main Results:

T. basicola differentiates infection structures upon contact with living root hairs.
Host cells exhibit cytoplasmic aggregation and callose deposition (bell-shaped collar) around infection sites.
The fungus penetrates living root hairs via threadlike hyphae, forming sickle-shaped intracellular hyphae and colonizing the cell, leading to limited host survival.

Conclusions:

T. basicola establishes a hemibiotrophic parasitic relationship, initially biotrophic, leading to colonization and eventual host cell limitation.
Infection of dead root hairs does not lead to sustained association or development of infection structures.
The observed interaction strategy is characteristic of hemibiotrophic fungi.