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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
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The effect of fecal turbidity on norovirus detection by reverse transcriptase polymerase chain reaction

Kristie J Witlox1, Theo Karapanagiotidis, Leesa D Bruggink

  • 1Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia.

Diagnostic Microbiology and Infectious Disease
|October 24, 2008
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...