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Updated: Jun 28, 2026
Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice
Published on: July 8, 2020
Microarray analysis of micro-ribonucleic acid expression in primary immunoglobulin A nephropathy
Yong Dai1, Weiguo Sui, Huijuan Lan
1Clinical Medical Research Center, The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Guangdong Province, China.
Objective:
To explore the relationship between immunoglobulin A nephropathy (IgAN) and microRNA (miRNA).
Methods:
We analyzed the miRNA expression profiles in renal biopsies from 11 IgAN patients and 3 controls at the Kidney Transplantation and Hemo Purification Center of 181 Hospital, China, from May to October 2007, using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences.
Results:
This study identified 132 miRNAs in renal samples, of which 35 miRNAs up-regulated in IgAN biopsies. The chip results were confirmed by northern blot analysis and by quantitative real-time polymerase chain reaction (RT-PCR) tests.
Conclusion:
Our study may help clarify the molecular mechanisms involved in the pathogenesis of IgAN, and miRNAs potentially serve as a novel diagnostic biomarker of IgAN.
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