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IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.

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Related Experiment Video

Updated: Jun 28, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

Completely automated open-path FT-IR spectrometry.

Peter R Griffiths1, Limin Shao, April B Leytem

  • 1Department of Chemistry, University of Idaho, Moscow, ID, 83843-2343, USA. pgriff@uidaho.edu

Analytical and Bioanalytical Chemistry
|October 24, 2008
PubMed
Summary
This summary is machine-generated.

Open-path Fourier-transform infrared (OP/FT-IR) spectrometry advancements overcome software limitations for atmospheric analysis. New methods enable precise trace gas concentration measurements without complex background spectra, improving atmospheric monitoring capabilities.

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High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
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Last Updated: Jun 28, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
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High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
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High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Open-path Fourier-transform infrared (OP/FT-IR) spectrometry has been available for over 20 years.
  • Commercial instrument software limitations have hindered its widespread adoption for atmospheric analysis.
  • Existing methods require complex background spectrum acquisition, complicating trace gas analysis.

Purpose of the Study:

  • To describe the state-of-the-art hardware and software for contemporary OP/FT-IR spectrometers.
  • To present laboratory-developed advancements that overcome previous instrument limitations.
  • To enable more efficient and accurate atmospheric trace molecule concentration determination.

Main Methods:

  • Implemented a simplified "short path-length" background spectrum acquisition instead of single-beam background spectra.
  • Replaced classical least-squares regression (CLS) with partial least-squares regression (PLS) for concentration calculations.
  • Utilized wavelet transforms for automatic baseline correction and incorporated a novel detector nonlinearity correction method.

Main Results:

  • Successfully overcame limitations associated with water vapor and carbon dioxide absorption features.
  • Achieved accurate atmospheric species concentration measurements using OP/FT-IR spectra.
  • Demonstrated continuous, automated monitoring of atmospheric species concentrations over extended periods (hours) at 1-minute intervals.

Conclusions:

  • Advanced OP/FT-IR techniques offer a robust solution for real-time atmospheric monitoring.
  • The developed methods significantly enhance the usability and accuracy of OP/FT-IR for environmental analysis.
  • Automated, long-term atmospheric species concentration monitoring is now feasible with minimal operator intervention.