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Purification to homogeneity of bovine prolactin by high-performance ion-exchange chromatography
A Berrini1, V Borromeo, C Secchi
1Istituto di Fisiologia Veterinaria e Biochimica, Università di Milano, Italy.
Journal of Chromatography
|June 28, 1991
Abstract:
Homogeneous bovine prolactin (bPRL) has been obtained using a procedure based on high-performance anion-exchange chromatography. The procedure enables up to 6 mg of 99.4% pure bPRL to be obtained per hour, with a recovery of 32.4%. The purity of the protein was checked by N-terminal sequencing and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The highly purified bPRL obtained with this method is suitable for complete structural and immunochemical studies.