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Updated: Jun 28, 2026

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
Published on: February 8, 2016
Differential development of ectopic testes based on their location in rats
Takeshi Shono1, Tsuyoshi Kondo, Sachiyo Suita
1Department of Pediatric Surgery, Reproductive and Developmental Medicine, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. shono@pedsurg.med.kyushu-u.ac.jp
Objective:
In order to investigate the effect of location on the development of ectopic testes, the relationship between the degree of degenerative change in ectopic testes and their original position was examined in rat models of cryptorchidism.
Materials And Methods:
Unilateral ectopic testes were created by excising the future scrotal skin on either the right or left side in newborn rats. At 60 days of age, the position of the testes was examined, the testicular weight and seminiferous tubular diameter (STD) were measured, and spermatogenesis was evaluated using the Johnsen score.
Results:
All the rats showed maldescended testes on the operated side, with the testes descending into the superficial inguinal pouch, the perineum or the transverse contralateral scrotum. The testicular weight, STD and Johnsen score were all significantly lower for the superficial inguinal testes than for either the perineal or contralateral scrotal testes.
Conclusion:
These findings suggest that the degree of testicular degeneration depends on the position of the ectopic testes; the higher the testicular location, the greater the histological changes.
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