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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: Jun 28, 2026

Performing Custom MicroRNA Microarray Experiments
07:04

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

OligoArrayDb: pangenomic oligonucleotide microarray probe sets database.

Jean-Marie Rouillard1, Erdogan Gulari

  • 1Chemical Engineering Department, University of Michigan, 2300 Hayward Street, Ann Arbor, MI 48109, USA. jmrouill@umich.edu

Nucleic Acids Research
|October 25, 2008
PubMed
Summary
This summary is machine-generated.

OligoArrayDb provides pangenomic oligonucleotide microarray probe sets for over 600 sequenced organisms, enabling custom microarray experiments. This database offers extensive probe coverage and specificity for genes across diverse microbial life.

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Last Updated: Jun 28, 2026

Performing Custom MicroRNA Microarray Experiments
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Published on: October 28, 2011

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09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

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13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Commercial microarray platforms often lack probe sets for non-model organisms.
  • There is a need for accessible tools to design custom microarrays for a wide range of sequenced genomes.

Purpose of the Study:

  • To introduce OligoArrayDb, a comprehensive database of pangenomic oligonucleotide microarray probe sets.
  • To facilitate custom microarray experiments for organisms not covered by commercial arrays.

Main Methods:

  • Development of a database containing probe sets for over 600 sequenced genomes, primarily archaea and bacteria.
  • Inclusion of probe sequences, gene annotations, and specificity predictions.
  • Weekly updates to incorporate newly available sequenced genomes.

Main Results:

  • OligoArrayDb offers an average of 2.8 probes per gene for over 600 organisms.
  • Approximately 98% of annotated genes have at least one probe.
  • Probes demonstrate predicted specificity to their targets in over 94% of cases.

Conclusions:

  • OligoArrayDb provides a valuable resource for researchers needing custom microarray probes for diverse microbial genomes.
  • The database supports custom microarray fabrication, expanding research possibilities in genomics and molecular biology.