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Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
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Nuclear Protein Sorting01:34

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piRNA - Piwi-interacting RNAs02:57

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Anaphase Promoting Complex00:50

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Related Experiment Video

Updated: Jun 28, 2026

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
07:54

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Published on: August 19, 2014

PCNA is ubiquitinated by RNF8.

Sufang Zhang1, Jennifer Chea, Xiao Meng

  • 1Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA.

Cell Cycle (Georgetown, Tex.)
|October 25, 2008
PubMed
Summary

RNF8, a DNA Damage Response E3 ligase, also ubiquitinates PCNA, a key protein in DNA Damage Tolerance. This suggests RNF8 plays a role in both DNA repair pathways.

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Detection of Protein Ubiquitination
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Last Updated: Jun 28, 2026

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
07:54

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Published on: August 19, 2014

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)
09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

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Detection of Protein Ubiquitination
09:00

Detection of Protein Ubiquitination

Published on: August 19, 2009

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • Ubiquitination of PCNA is crucial for the DNA Damage Tolerance (DDT) pathway, enabling bypass of DNA lesions during S-phase.
  • The DDT pathway utilizes mono- and polyubiquitination of PCNA for translesion synthesis and template switching.
  • DNA damage from ionizing radiation (IR) triggers the DNA Damage Response (DDR), involving proteins like RNF8.

Purpose of the Study:

  • To investigate the role of RNF8 in PCNA ubiquitination.
  • To determine if RNF8 participates in the DNA Damage Tolerance pathway.

Main Methods:

  • In vitro ubiquitination assays using RNF8, PCNA, and various ubiquitin-conjugating enzymes.
  • Depletion of RNF8 in cells followed by analysis of PCNA ubiquitination after UV or MNNG treatment.

Main Results:

  • RNF8 catalyzes mono-ubiquitination of PCNA with UbcH5c and polyubiquitination with Ubc13/Uev1a in vitro.
  • RNF8-dependent PCNA ubiquitination was observed in vivo, as RNF8 depletion reduced UV- and MNNG-induced PCNA ubiquitination.

Conclusions:

  • RNF8, a key DDR E3 ligase, can perform PCNA ubiquitination reactions similar to those in the DDT pathway.
  • These findings indicate a potential role for RNF8 in the DNA Damage Tolerance pathway, in addition to its known function in the DDR.