Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synergy of a Low-methionine Diet and Reduced-dose Cisplatinum Eradicates Experimental Lung-cancer Bone Metastases in a Nude-mouse Model.

In vivo (Athens, Greece)·2026
Same author

Direct comparison of efficacy of combining ivermectin versus five first-line chemotherapy drugs with recombinant methioninase against colon-cancer cells.

Frontiers in oncology·2026
Same author

The Combination of Methionine Adenosyltransferase 2A (MAT2A) Inhibitor AG-270 and Recombinant Methioninase Is Not Cancer-selective in a Co-culture Model of Colon Cancer Cells and Normal Fibroblasts.

Cancer diagnosis & prognosis·2026
Same author

Chemotherapy in Combination With Methionine Restriction Induced A Complete Response in a Patient With Recurrent Metastatic Tongue Squamous-Cell Carcinoma.

Cancer diagnosis & prognosis·2026
Same author

Sulfasalazine an Inhibitor of System x<sub>C</sub> <sup>-</sup> (Cystine/glutamate Antiporter), Combined With Recombinant Methioninase, Inhibits Both Cancer and Normal Cells, Suggesting Lack of Cancer Selectivity of Cysteine Restriction.

Anticancer research·2026
Same author

The Glucose and Glutamine Requirements of Cancer and Normal Cells Do Not Distinguish Them, in Contrast to Their Methionine Requirement, Suggesting the Warburg Effect Is Not a Cancer Paradigm.

Anticancer research·2026

Related Experiment Video

Updated: Jun 28, 2026

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells
07:37

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Published on: November 17, 2017

Enzymatic assay for total plasma Cys.

Qinghong Han1, Robert M Hoffman

  • 1AntiCancer, Inc., 7917 Ostrow Street, San Diego, CA 92111, USA.

Nature Protocols
|October 25, 2008
PubMed
Summary

This study presents a new enzymatic assay to measure total cysteine (tCys) in plasma. This rapid and sensitive method can help detect elevated tCys levels in patients with vascular or kidney disease.

Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Enzymology

Background:

  • Elevated plasma total cysteine (tCys) concentrations are observed in patients with vascular disease and end-stage renal disease.
  • Accurate measurement of tCys is crucial for understanding disease pathology and progression.
  • Existing methods for tCys measurement may be time-consuming or require complex procedures.

Purpose of the Study:

  • To develop and validate a novel, nonradioactive, enzymatic colorimetric assay for the precise and sensitive quantification of plasma tCys.
  • To provide a homogeneous assay that avoids the need for sample separation methods.
  • To establish a rapid and reliable method for tCys measurement in clinical settings.

Main Methods:

  • Utilized recombinant enzymes methionine alpha,gamma-lyase (rMETase) and S-adenosylhomocysteine hydrolase (rSAHH).

More Related Videos

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
10:12

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

Published on: August 8, 2013

Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
07:14

Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Published on: June 16, 2023

Related Experiment Videos

Last Updated: Jun 28, 2026

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells
07:37

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Published on: November 17, 2017

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
10:12

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

Published on: August 8, 2013

Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
07:14

Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Published on: June 16, 2023

  • Employed a colorimetric detection method measuring hydrogen sulfide (H(2)S) produced from Cys using N,N-dibutylphenylenediamine (DBPDA).
  • The assay is homogeneous, requiring no prior separation steps, and takes 3-4 hours to complete.

    • Main Results:

    • The developed assay is nonradioactive, precise, rapid, and sensitive for plasma tCys determination.
    • The assay successfully quantifies tCys by measuring H(2)S generated exclusively from Cys via enzymatic reactions.
    • Homogeneous nature simplifies the procedure by eliminating separation steps.

    Conclusions:

    • A novel enzymatic colorimetric assay for plasma tCys has been successfully developed.
    • This assay offers a sensitive, precise, and rapid method for tCys quantification.
    • The assay's simplicity and speed make it suitable for clinical applications, particularly for patients with vascular and renal diseases.