Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Staff experiences of percutaneous tracheostomy in intensive care: challenges, complications and potential solutions.

Anaesthesia reports·2026

Same author

Pre- and post-cranioplasty hydrocephalus in patients following decompressive craniectomy for ischemic stroke: a systematic review and meta-analysis.

Neurosurgical review·2025

Same author

The Impact of Inoculation of Two Strains of Rhizobacteria on Radionuclide Transfer in Sesbania Grandiflora.

Bulletin of environmental contamination and toxicology·2024

Same author

Prevention and screening during the COVID-19 pandemic: qualitative findings from the BETTER WISE project.

BMC primary care·2023

Same author

D-Dimer May be a Prognostic Hematological Marker for COVID-19: A Retrospective Case Analysis.

Mymensingh medical journal : MMJ·2021

Same author

Sub-continental Atmosphere and Inherent Immune System may have Impact on Novel Corona Virus' 2019 (nCovid-19) Prevalence in South East Asia.

Mymensingh medical journal : MMJ·2020

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

MicroRNA profile analysis of human prostate cancers.

A W Tong¹, P Fulgham, C Jay

¹Gradalis, Inc., Dallas, TX, USA. alext@baylorhealth.edu

Cancer Gene Therapy

|October 25, 2008

Summary

This study reveals that altered microRNA (miRNA) expression is linked to prostate cancer development and early relapse. Restoring specific miRNAs inhibited cancer cell growth, suggesting potential therapeutic applications.

More Related Videos

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

Published on: May 16, 2012

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

Related Experiment Videos

Last Updated: Jun 28, 2026

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

Published on: May 16, 2012

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Prostate cancer progression involves complex molecular changes.
MicroRNAs (miRNAs) are key regulators of gene expression implicated in oncogenesis.
Understanding miRNA dysregulation in prostate cancer is crucial for identifying biomarkers and therapeutic targets.

Purpose of the Study:

To investigate the microRNA (miRNA) expression profile in prostate cancer tissues.
To correlate miRNA expression patterns with tumor stage, relapse, and oncogenesis.
To explore the functional role of specific miRNAs in prostate cancer cell growth.

Main Methods:

Analysis of miRNA expression in 40 prostatectomy specimens using high-throughput liquid-phase hybridization (mirMASA) with 114 miRNA probes.
Paired analysis of malignant and non-involved tissue areas.
Validation of miRNA expression using quantitative reverse transcription (qRT)-PCR.
Functional assays involving ectopic miRNA expression in LNCaP cancer cells.

Main Results:

Five miRNAs (miR-23b, -100, -145, -221, -222) were significantly downregulated in malignant prostate tissues.
Lowered expression of miR-23b, -145, -221, and -222 was validated by qRT-PCR.
Ectopic expression of these miRNAs inhibited LNCaP cancer cell growth.
A distinct miRNA expression profile was observed in patients with early relapse compared to non-relapse patients.
Increased expression of miR-135b and miR-194 showed a trend in early relapse patients.

Conclusions:

Altered miRNA expression signatures are associated with prostate oncogenesis.
Specific miRNA expression patterns may predict early disease relapse.
Reconstitution of miRNA expression holds potential for experimental prostate cancer therapy.