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Effect of 7Li radiation on endogenous hormonal level on developing cotton fiber
Kunjal Bhatt1, Asiti Sarma, Vrinda Thaker
1Department of Biosciences, Saurashtra University, Rajkot 360 005.
Abstract:
Influence of radiation doses (7Li) on cellular metabolism, specially endogenous hormonal level, was studied in monolayer of cotton fibers. Changes in endogenous phytohormone level were determined with two different fluences of 7Li equivalent to radiation doses of 1 Gy and 4 Gy. To estimate the endogenous indole-3-acetic acid (IAA), phenoxy acetic acid (PAA), and abscisic acid (ABA) levels, indirect ELISA was performed with the help of antibodies raised against each hormone. In samples at later stage, dose dependent response was apparent in PAA. Results showed that in vivo content of each hormone increased with radiation treatment except ABA.
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